Native cytoplasmic volume in HeLa cell

Range maximum 4.4 pL: median 1.6±0.7 pL pL
Organism Human Homo sapiens
Reference Guillaume-Gentil O et al., Tunable Single-Cell Extraction for Molecular Analyses. Cell. 2016 Jul 14 166(2):506-16. doi: 10.1016/j.cell.2016.06.025. p.508 right column 3rd paragraphPubMed ID27419874
Method P.507 left column 2nd paragraph: "Using fluidic force microscopy (FluidFM), [investigators] recently established an effective protocol for the minimally invasive insertion, stabilization, and withdrawal of the tip of a microchanneled probe inside a cell for solute injection (Guillaume-Gentil et al., 2013, Guillaume-Gentil et al., 2014 and Meister et al., 2009). Because the FluidFM is mounted on top of an inverted optical microscope, the cells of interest can be readily observed during manipulation in real time. The process relies on AFM to accurately and gently drive the probe through the cell membrane. In this study, [they] developed and explored a platform for the extraction of tunable amounts of intracellular fluid from live cells and the delivery of the withdrawn cell contents to a variety of analytical techniques."
Comments P.508 right column 3rd paragraph: "Considering the native cytoplasmic volumes measured in the pool of HeLa cells (maximum 4.4 pl median 1.6 ± 0.7 pl Figure 3A), the observed loss in viability after extraction was most likely related to the complete removal of the cytoplasmic content."
Entered by Uri M
ID 112928