Endogenous enzyme levels in HeLa cells

Range ~40,000 (or 33.5 fmol) copies/cell
Organism Human Homo sapiens
Reference Guillaume-Gentil O et al., Tunable Single-Cell Extraction for Molecular Analyses. Cell. 2016 Jul 14 166(2):506-16. doi: 10.1016/j.cell.2016.06.025. p.511 right column bottom paragraphPubMed ID27419874
Primary Source Nagaraj N et al., Deep proteome and transcriptome mapping of a human cancer cell line. Mol Syst Biol. 2011 Nov 8 7: 548. doi: 10.1038/msb.2011.81.PubMed ID22068331
Method Primary source abstract: "Online liquid chromatography coupled to high-resolution MS and MS/MS."
Comments p.511 right column bottom paragraph: "First, [investigators] selected a beta-galactosidase (β-gal) assay using the fluorogenic substrate fluorescein di-β-D-galactopyranoside (FDG). β-gal is widely applied as reporter enzyme to assess the transcriptional activity of promoters due to relatively low endogenous enzyme levels in mammalian cells (approximately 40,000 copies or 33.5 fmol in a HeLa cell [primary source])."
Entered by Uri M
ID 112929