Nuclear and intrachromosomal volume of HeLa cells uninfected and infected by herpes simplex virus

Range Table - link
Organism Human Homo sapiens
Reference Monier K, Armas JC, Etteldorf S, Ghazal P, Sullivan KF. Annexation of the interchromosomal space during viral infection. Nat Cell Biol. 2000 Sep2(9):661-5PubMed ID10980708
Method Researchers quantitatively monitored chromatin organization and chromosome dynamics in living cells after HSV-1 infection by using a fusion of histone H2B and green fluorescent protein (H2B–GFP)(ref 15). For volume measurements, confocal sections of individual nuclei from H2B–GFP HeLa cells either not infected or infected with HSV-1 were processed and analysed with 3-D image-analysis software (Ana3D© Y. Usson).
Comments Table gives volumes of nuclei of non-infected and infected Hela cells 8 and 9.5 hours after infection. Researchers report direct quantitative analysis of the global structural perturbations of interphase chromosome and interchromosome domain distribution caused by infection with herpes simplex virus-1 (HSV-1).
Entered by Uri M
ID 104715