Comparison of growth rates and relative resistance levels to four different “Serine-type” PSII herbicides

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Organism Green algae Chlamydomonas reinhardtii
Reference Britta Förster, Peter B. Heifetz, Anita Lardans, John E. Boynton, & Nicholas W. Gillham, Herbicide Resistance and Growth of D1 Ala251 Mutants in Chlamydomonas, Zeitschrift für Naturforschung C, A Journal of Biosciences, Volume 52, Issues 9-10 pp.654-664 (1997) DOI: link p.662 table II
Method Abstract: "[Investigators] elucidated the effects of substituting seven amino acids for Ala at residue 251 of the Chlamydomonas reinhardtii D1 protein on herbicide resistance and photoautotrophic growth...Sensitive and rapid microtiter assays were employed to compare herbicide resistance and photoautotrophic growth in the various mutants." P.656 left column 3rd paragraph: "Mutant and wildtype strains were grown photoautotrophically in HS (high salt) medium or mixotrophically in HSHA (high salt high acetate) medium for physiological experiments as described by Lers et al. (1992). Photoautotrophic cultures were incubated under VLL (very low light, 33 µmol/m^2/s), LL (low light, 70 µmol/m^2/s), and HL (high light, 600 µmol/m^2/s). Media were prepared as described in Harris (1989) except that the phosphate stock solution was autoclaved separately and added to cool sterile medium to avoid cloudy precipitates that interfere with A 750 measurements. For spot tests and strain maintenance (1.5% w/v) agar solidified media (HS, HSHA) were used at 25˚C in VLL, LL, or HL. Antibiotics and herbicides were added from concentrated stock solutions (in ethanol) to the liquid or solid media after autoclaving."
Comments P.661 right column 2nd paragraph: "[Investigators] find that substitution of Ile or Val for Ala251 results in equivalent phenotypic changes whereas substitution of Leu increases both herbicide resistance and impairment of autotrophic growth relative to the Ile and Val mutations (Table II)."
Entered by Uri M
ID 116798