Growth rate at the beginning of growth phase, cytosolic pH=7.0

Value 0.48 hour^-1
Organism Budding yeast Saccharomyces cerevisiae
Reference Orij R et al., Genome-wide analysis of intracellular pH reveals quantitative control of cell division rate by pH(c) in Saccharomyces cerevisiae. Genome Biol. 2012 Sep 10 13(9):R80. doi: 10.1186/gb-2012-13-9-r80. p.2 left column bottom paragraphPubMed ID23021432
Method "[Researchers] assessed pHc in growing yeast cultures under batch conditions to reveal that pHc is not constant during growth. pHc was neutral in the beginning of the exponential growth phase, and then gradually dropped approximately 0.3 pH units in mid-to late exponential phase, before glucose was depleted. Upon glucose depletion pHc decreased to 5.5 (Figure 1a)."
Comments "[Researchers] determined that the gradual reduction of pHc [cytosolic pH] from 7 to 6.7 during growth of the culture was a response to changes in the cellular environment, rather than a property of the cultured cells themselves: [researchers] took cell and culture supernatant samples from the beginning of the growth phase (where pHc was 7.0 and the specific growth rate 0.48 h^-1) and at the end of the growth phase, just prior to glucose depletion, when the pHc and growth rate were decreased." equivalent to doubling time of 1.44 hours
Entered by Uri M
ID 110913