Sources that lipid carbon is derived from assuming that cellular lipids are derived from only glucose, glutamine, and extracellular lipids

Range from exogenous sources 60%-70%: from glucose 20%-30%: from glutamine ~5% %
Organism Mammalian tissue culture cell
Reference Hosios AM et al., Amino Acids Rather than Glucose Account for the Majority of Cell Mass in Proliferating Mammalian Cells. Dev Cell. 2016 Mar 7 36(5):540-9. doi: 10.1016/j.devcel.2016.02.012 p.544 right column top paragraphPubMed ID26954548
Method P.544 left column: "To assess the contribution of lipid biosynthesis from glucose relative to lipid scavenging from the environment, [investigators] measured the increase in glucose incorporation into the lipid fraction when proliferating cells were deprived of exogenous lipids. Cells were cultured in serum stripped of lipids by non-denaturing organic extraction (Figure S3F)."
Comments P.544 left column: "Finally, the difference in the proportion of cell mass derived from glucose when cells are grown with or without serum fatty acids corresponds well with the measured contribution of palmitate and oleate carbon to cell mass when these fatty acids are present (Figures 3E and S3G). This corroborates the notion that these two fatty acids are the predominant mass contributors from exogenous lipids and argues that lipid mass in these cells is obtained from a combination of scavenging and de novo synthesis from glucose. Assuming that cellular lipids are derived from only glucose, glutamine, and extracellular lipids, the above data suggest that 60%–70% of lipid carbon is derived from exogenous sources, while 20%–30% is derived from glucose and ∼5% from glutamine."
Entered by Uri M
ID 116051