Contribution of cholesterol to apical membrane lipids of MDCK cell

Range ~45 Figure link - link mol%
Organism Mammalian tissue culture cell
Reference Gerl et al., Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane. J Cell Biol. 2012 Jan 23 196(2):213-21 p.215 left column bottom paragraph and fig.2BPubMed ID22249292
Method "[Researchers] used a shotgun lipidomics approach that combines high mass accuracy and resolution mass spectrometry (MS) and a two-step extraction procedure for optimal recovery of polar lipids with the use of internal standard mixtures for absolute quantification of 18 lipid classes and 419 lipid species..."
Comments "As expected, [researchers] found a high cholesterol content in the AP (apical membrane) cholesterol comprising ~45 mol% of all apical lipids was the most abundant lipid species."
Entered by Uri M
ID 108435