| Comments |
P.707 left column 2nd paragraph: "In typical oxygen-uptake experiments [investigators] see that indeed cells have a range of dioxygen utilization (Table 1). U937 cells (non-Hodgkin lymphoma) use oxygen at a rate of ≈ 4 amol/cell/s, whereas PC-3 cells (prostate adenocarcinoma) use oxygen at 10 times this rate, 45 amol/cell/s. Thus, [they] might expect these cells to have quite different strategies to maintain an appropriate redox environment with varying metabolic demands (normal and pathological). The rates of oxygen consumption, using a Clark electrode, presented in Table 1 are for cells while in suspension. U937 cells grow in suspension culture, however, PC-3 cells grow as adherent cells (monolayer). For cells that normally grow in a monolayer an O2 uptake measurement when in suspension may not be an accurate estimate of their rate of oxygen uptake in the usual cell culture setting, but may establish reasonable ranges, albeit corroboration by other approaches may be needed." amol=attomol=10^−18 moles |