| Range |
dermal fibroblasts 1.19×10^-17: bone marrow derived stromal cells 7.91×10^-18 mol/cell/sec
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| Organism |
Human Homo sapiens |
| Reference |
Streeter I, Cheema U. Oxygen consumption rate of cells in 3D culture: the use of experiment and simulation to measure kinetic parameters and optimise culture conditions. Analyst. 2011 Oct 7 136(19):4013-9. doi: 10.1039/c1an15249a abstractPubMed ID21804989
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| Method |
Abstract: "[Investigators] have cultured two cell types in 3D native collagen type I scaffolds, and measured the O(2) tension at specific locations within the scaffold." P.4014 right column 4th paragraph: "Fibre-optic oxygen probes (Oxford Optronix, UK) were inserted into the centre of the 3D constructs to place the measuring tip halfway along their long axis (Fig. 1)." |
| Comments |
P.4016 right column 2nd paragraph: "For HDFs [Human dermal fibroblasts], the average value of Vm [oxygen consumption rate constant] over the time period shown in Fig. 2b is 1.19×10^−17 mol/cell/sec. For HBMSCs [Human bone marrow-derived stem cells], the average value of Vm over the time period shown in Fig. 3b is 7.91×10^−18 mol/cell/sec." P.4018 right column 5th paragraph: "From [investigators’] simulations, [they] find that the number of HDFs (Vm = 1.19×10^−17 mol/cell/sec) in the construct should not exceed 1.80 million, which is a cell density of 2.00×10^7cells/cm^3. Similarly, the number of HBMSCs (Vm = 7.91×10^−18 mol/cell/sec) in the construct should not exceed 2.70 million, which is a cell density of 3.00×10^7cells/cm^3." |
| Entered by |
Uri M |
| ID |
115116 |