| Value |
0.97
unitless
Range: ±0.56 unitless
|
| Organism |
Ciliate Paramecium caudatum |
| Reference |
Katsu-Kimura Y, Nakaya F, Baba SA, Mogami Y. Substantial energy expenditure for locomotion in ciliates verified by means of simultaneous measurement of oxygen consumption rate and swimming speed. J Exp Biol. 2009 Jun212(Pt 12):1819-24. doi: 10.1242/jeb.028894. p.1821 left column top paragraphPubMed ID19482999
|
| Method |
"In this paper, [researchers] will present the energy expenditure of
Paramecium in close relation to its swimming activity. For this
purpose, paramecia were confined in a small volume of the chamber
(<1 ml) and the oxygen consumption rate and the swimming speed
were measured simultaneously from the same specimens. Oxygen
consumption was measured by means of an optic fluorescence
oxygen sensor (Okubo et al., 2008). Because this sensor has proved
not to alter the amount of dissolved oxygen unlike oxygen electrodes,
which consume a substantial amount of oxygen during the
measurement procedure, it is ideal for measuring the oxygen
concentration in a small volume of a sample. Swimming speed was measured from the recording obtained by the optical slice method
(Kato et al., 2003)." |
| Comments |
"The VO2 [rate of oxygen uptake] of the cells in the experimental chamber (Fig. 1) while being stirred continually, by a small magnetic bar put into the cell
suspension, was not significantly different from that measured after
stirring had stopped and vice versa. Stirring and post-stirring
measurements were done 5–10 min after the stirring was ‘on’ and ‘off’, respectively. The ratio of the rate with stirring to that without
stirring was 0.97±0.56 (±s.d.) and was not significantly different
from 1.0 (N=8, P=0.87)." |
| Entered by |
Uri M |
| ID |
110785 |