Mutation rate

Range wild type ~0.0022: mutH mutant ~0.32 mutation/hour
Organism Bacteria Escherichia coli
Reference Lydia Robert, Jean Ollion, Jerome Robert, Xiaohu Song, Ivan Matic, and Marina Elez, Mutation dynamics and fitness effects followed in single cells, Science 16 Mar 2018: Vol. 359, Issue 6381, pp. 1283-1286 DOI: 10.1126/science.aan0797 p.1284 middle column bottom paragraph
Primary Source [11] Lee H, Popodi E, Tang H, Foster PL. Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing. Proc Natl Acad Sci U S A. 2012 Oct 9 109(41):E2774-83. doi: 10.1073/pnas.1210309109. [14] Materials and methods and supplementary text link PubMed ID22991466
Method P.1283 right column top paragraph: "For real-time detection of mutations in single living cells, [investigators] developed a mutation visualization (MV) experiment. [They] also designed a microfluidic MA (µMA) experiment, which allowed [them] to track fitness in the complete absence of natural selection in a high-throughput fashion during the accumulation of mutations."
Comments P.1284 middle column bottom paragraph: "Using MV, [investigators] estimated mutation rates for E. coli wild-type (WT) (~0.0022 mutation/hour) and mutH mutant (~0.32 mutation/hour) strains (primary source 14), which agree with estimations from wholegenome sequencing of MA [mutation accumulation] lines of WT and mismatch repair–deficient cells (primary source 11)."
Entered by Uri M
ID 114861