Spontaneous mutation rate

Range ~0.001 (or 2.2×10^−10 mutations/nucleotide/generation) mutations/genome/generation
Organism Bacteria Escherichia coli
Reference Lee H, Popodi E, Tang H, Foster PL. Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing. Proc Natl Acad Sci U S A. 2012 Oct 9 109(41):E2774-83. doi: 10.1073/pnas.1210309109. abstract, p.E2775 left column 2nd paragraph & table 1PubMed ID22991466
Method “The wild-type strain [researchers] used for the MA [mutation-accumulation] protocol is a prototrophic derivative of the reference E. coli strain, MG1655. The parameters of a two-part MA experiment with this strain are given in Table 1. In the first part (designated “wild-type 3K”), MA lines were passaged for about 3,000 generations before sequencing in the second part (designated “wild-type 6K”), unsequenced lines continued to be passaged for an additional 3,000 generations and then were sequenced. Surprisingly, the 6K lines accumulated more mutations per generation than did the 3K lines. Thus, [researchers] have two estimates of the spontaneous mutation rate that differ by 30% but are within 95% confidence limits (CL) of each other.”
Comments “Both parts of the experiment gave results that appear to be random and unbiased (see below) thus, the best estimate of the spontaneous mutation rate is the midpoint of these limits, 2.2×10^-10 mutations per nucleotide per generation or 1.0×10^-3 mutations per genome per generation (Table 1). This rate is about threefold lower than Drake’S value of 3–4×10^-3 per genome per generation for all DNA-based microbes (PMID 1831267, PMID 19543367).”
Entered by Uri M
ID 111339