| Reference |
Zavřel, T., Očenášová, P., Červený, J (2017): Phenotypic characterization of Synechocystis sp. PCC 6803 substrains reveals differences in sensitivity to abiotic stress. PLoS ONE 12(12): 1-21, DOI: 10.1371/journal.pone.0189130PubMed ID29216280
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| Method |
P.6 bottom paragraph: "10 ml of culture suspension was collected in 15-ml falcon tubes and centrifuged (4 000 g, 10 min, 4˚C), supernatant was discarded and pellet was stored in -80˚C for 2 months until further processing. After thawing on ice, the pellets were resuspended in PBS buffer (pH 7.4) and centrifuged (4 000 g, 10 min,4˚C). Supernatant was partially discarded and the cells were transferred to Eppendorf tubes on ice. The Eppendorf tubes were centrifuged again (15 000 g, 10 min, 4˚C), supernatant was discarded and the tubes were put to -80˚C for 30 min. Frozen samples were lyophilized overnight. After lyophilization, glass beads were added to dry cellular pellets and the samples were homogenized on Silamat S6 homogenizer (Ivoclar Vivadent AG, Schann, LI) for 30 sec. After homogenization, PBS buffer (4˚C) was added to the tubes for the phycobilisomes extraction, the samples were vortexed for 3 sec and put on ice in the dark for 30 min. After extraction, the samples were centrifuged (15 000 g, 10 min and 4˚C) and concentration of phycobilisomes was measured with the use of spectrophotometer UV 2600 (Shimadzu Scientific Instruments, Inc., Columbia, MD, USA)." |