Carbohydrates content

Value 28.4 % of dry weight Range: 16.4 - 40.4 % of dry weight
Organism Cyanobacteria Synechocystis PCC 6803
Reference Zavřel, T., Očenášová, P., Červený, J (2017): Phenotypic characterization of Synechocystis sp. PCC 6803 substrains reveals differences in sensitivity to abiotic stress. PLoS ONE 12(12): 1-21, DOI: 10.1371/journal.pone.0189130PubMed ID29216280
Method P.7 2nd paragraph: "After harvesting, 1 ml of cellular suspension was centrifuged (20 000 g, 15 min, 25˚C) and the pellet was resuspended in 1 ml of distilled water. 8 x 30 μl of each sample was transferred to 96-well plate, and 30 μl of 5% phenol, followed by 150 μl of 96% H2SO4 was added to each well (after each step, the suspension was mixed by gentle pipetting). The plates were covered with the original plate lid and the samples were incubated at laboratory temperature for 60 min. The concentration of cellular saccharides was measured by Multiskan™ GO Microplate Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) at 490 nm, using D-glucose as a standard."
Comments Three substrains of Synechocystis sp. PCC 6803 cultivated in photobioreactors in turbidostat regime under low and high light intensities. From 1st author, Zavřel: "Calculated from the measured data, not shown in the paper"
Entered by Tomas Zavrel
ID 114567