Protein content

Range 32 - 57 % of dry weight
Organism Cyanobacteria Synechocystis PCC 6803
Reference Zavřel, T., Očenášová, P., Červený, J (2017): Phenotypic characterization of Synechocystis sp. PCC 6803 substrains reveals differences in sensitivity to abiotic stress. PLoS ONE 12(12): 1-21, DOI: 10.1371/journal.pone.0189130 p.16 3rd paragraphPubMed ID29216280
Method P.7 2nd paragraph: "Content of cellular proteins was measured by Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, St. Louis, MO, USA), using bovine serum albumin as a standard, and following instructions of the manufacturer."
Comments P.16 3rd paragraph: "The tested Synechocystis substrains varied also in growth rates (Fig 2), photosynthetic rates (Fig 3), cellular dry weight (Fig 4), proteins content (Fig G in S1 File) and carbohydrates content (Fig 4, Figs I and J in S1 File). Such variation on Synechocystis substrain level is reported for the first time here. The overall carbohydrates content (up to c. 40% of dry weight for GT-L substrain under high red light) was higher than reported previously in another Synechocystis substrain (up to c. 13%, [ref 31]). On the other hand, cellular protein content (32–57% of cellular dry weight for all three Synechocystis substrains tested here) was lower than reported previously (61–73%, [ref 31]), even though the photosynthetic rates measured here were comparable with the previous study (0.03–0.21 mol(O2)/mol(Chl)/s in this study vs. 0.066–0.081 mol(O2)/mol(Chl)/s in Touloupakis et al. (2015) [ref 31])." Three substrains of Synechocystis sp. PCC 6803 cultivated in photobioreactors in turbidostat regime under low and high white light.
Entered by Tomas Zavrel
ID 114566