| Range |
Env 7-10: Gag ≈2,400 Copies/virion
|
| Organism |
HIV |
| Reference |
Chojnacki J et al., Envelope glycoprotein mobility on HIV-1 particles depends on the virus maturation state. Nat Commun. 2017 Sep 15 8(1):545. doi: 10.1038/s41467-017-00515-6 p.2 left column top paragraph & bottom paragrahPubMed ID28916807
|
| Primary Source |
[1] Zhu P, et al. Electron tomography analysis of envelope glycoprotein trimers on HIV and simian immunodeficiency virus virions. Proc. Natl Acad. Sci. 2003 100: 15812–15817. doi: 10.1073/pnas.2634931100 [2] Chojnacki J, et al. Maturation-dependent HIV-1 surface protein redistribution revealed by fluorescence nanoscopy. Science. 2012 338 :524–528. doi: 10.1126/science.1226359 [3] Carlson L-A, et al. Three-dimensional analysis of budding sites and released virus suggests a revised model for HIV-1 morphogenesis. Cell Host Microbe. 2008 4 :592–599. doi: 10.1016/j.chom.2008.10.013PubMed ID14668432, 23112332, 19064259
|
| Method |
Primary source [1] electron tomography, primary source [2] fluorescence nanoscopy, primary source [3] electron tomography and scanning transmission electron microscopy |
| Comments |
P.2 left column top paragraph: "During its assembly, human immunodeficiency virus type 1 (HIV-1) incorporates 7–10 copies of the trimeric viral envelope surface glycoprotein Env [primary sources 1, 2] and ≈ 2400 copies of the main structural protein Gag [primary source 3]—a polyprotein that initially assembles into immature virus particles (Fig. 1a)." P.2 left column bottom paragraph: "In a conventional single-point STED-FCS [Stimulated emission depletion-fluorescence correlation spectroscopy] or even FCS measurements, which usually take a few seconds, the small pool of 7–10 tagged Env molecules on an individual virus would photobleach rapidly within less than a second, and therefore this measurement modality is unsuitable." |
| Entered by |
Uri M |
| ID |
114188 |