| Comments |
P.37 left column 3rd paragraph: "DRMs are preparations isolated from cells treated with different detergents (often 1% Triton X-100 at 4 °C). Such preparations have been commonly used to study lipid rafts [refs 65, 66]. [Investigators] have selected to compare the lipid data discussed above for exosomes and HIV particles with those reported for DRM preparations from KB and KBC cells [primary source 67], as these are the most detailed lipid analyses [they] are aware of for such preparations. As shown in Table 2, there are several similarities between these studies, although both DRM preparations and their parent cells contain higher amounts of PI [phosphatidylinositol], PE [phosphatidylethanolamine], PE O/P [PE ethers (alkyl or alkenyl)] (especially alkenyl ethers, often called plasmalogens), and considerably lower amounts of PS [phosphatidylserine]. A closer look at the composition of lipid species in the different preparations shows an upconcentration of the very-long-chain SM [sphingomyelin] species and no enrichment of PC 16:0/16:0 in both DRM preparations, in contrast to exosomes and HIV particles. Intriguingly, the ratio of lipids expected to be in the outer and inner leaflet is much lower for DRMs than could be expected for a bilayer structure, although these ratios are in the same range for the DRMs and HIV particles listed in Table 2." See notes beneath table. KB Cells="This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa." |