Yield of coenzyme Q10

Range Bacteria (E. coli) 0.45–3.63 mg/g DCW: Yeast (S. cerevisiae) 12.3 μg/g DCW
Organism Microbes
Reference Lee SQ, Tan TS, Kawamukai M, Chen ES. Cellular factories for coenzyme Q10 production. Microb Cell Fact. 2017 Mar 2 16(1):39. doi: 10.1186/s12934-017-0646-4. p.12 table 2PubMed ID28253886
Primary Source [85] Okada K, Kainou T, Matsuda H, Kawamukai M. Biological significance of the side chain length of ubiquinone in Saccharomyces cerevisiae. FEBS Lett. 1998 431 :241–4. [99] Lu W et al., Enhanced production of coenzyme Q10 by self-regulating the engineered MEP pathway in Rhodobacter sphaeroides. Biotechnol Bioeng. 2014 Apr111(4):761-9. doi: 10.1002/bit.25130.PubMed ID9708911, 24122603
Comments P.7 right column 3rd paragraph: "However, despite these efforts, CoQ10 levels in E. coli (0.45–3.63 mg/g DCW) still fall short of the levels produced by native producers (R. sphaeroides and A. tumefaciens) [primary source 99] (Table 2)." P.8 left column 2nd paragraph: "Unfortunately, similar to E. coli, S. cerevisiae natively produces CoQ6 not CoQ10 [ref 1]. Early attempts to delete the COQ1 gene in S. cerevisiae and replace it with DPS [decaprenyl diphosphate synthase] from G. suboxydans under the COQ1 promoter reportedly yielded 12.3 µg/g DCW [primary source 85]. However, DPS tends to require a heterodimer formation for proper function and, when expressed, may instead form dimers with native polyprenyl diphosphate synthases to produce products of differing lengths [ref 125] (Table 2)."
Entered by Uri M
ID 113505