Half-life of coenzymes in exponentially growing cells

Range Table - link min
Organism Microbes
Reference Hartl J, Kiefer P, Meyer F, Vorholt JA. Longevity of major coenzymes allows minimal de novo synthesis in microorganisms. Nat Microbiol. 2017 May 15 2 :17073. doi: 10.1038/nmicrobiol.2017.73 Supplementary information p.14 Supplementary table 1PubMed ID28504670
Method Abstract: "Here, [investigators] use dynamic 13C-labelling experiments to determine the half-life of major coenzymes of Escherichia coli." Supplementary information p.14 note beneath Supplementary table 1: "*Half -life (T50) values are obtained from fitting decaying isotopologue curves of respective coenzymes from dynamic labelling experiments (DLE). Data originates from single DLEs (available in Supplementary Data 1, 4, 5). The indicated fitting models were used: [E] = first order exponential decay, [W] = weibull. The indicated ± s.d. are the calculated from the standard deviations of the obtained parameters from curve fitting CoA [coenzyme A] was detected as acetyl-CoA and M2 isotopologue fraction was analysed to correct for acetyl-turnover. Similarly, M1 fraction was analysed for THF [Tetrahydrofuran] derivates to correct for C1-turnover. Where indicated, correction for salvage (CoA, NAD [Nicotinamide adenine dinucleotide]) was applied. Details on calculations, fittings are available in Supplementary Methods. Abbreviations: ND, not detected NQ, not quantified, Supp., supplemented with precursor."
Comments P.3 left column: "[Investigators] developed a tandem HRMS [high-resolution mass spectrometry] approach to determine the turnover of the catalytic core of CoA (that is, 4-phosphopantetheine) and found that it matched the generation time of E. coli (T50 of 60 ± 3 min, Fig. 3d, Supplementary Table 1), thus indicating that de novo synthesis is at a minimum." P.4 right column top paragraph: "Finally, as expected, the carbon backbone of ATP and guanosine triphosphate (GTP) had high exchange rates (T50 values of 4.7 ± 0.3 and 4.6 ± 0.3 min, Supplementary Fig. 2a, Supplementary Table 1). This can be explained by most of the nucleotides being incorporated into nucleic acids and does not indicate reduced stability of these building blocks."
Entered by Uri M
ID 113856