| Method |
P.3 left column 2nd paragraph: "Image Analysis-For intracranial volume and total brain volume, the images were transferred to a Linux workstation. Image processing was performed with Dr. View image analysis software (Asahi Kasei Joho System Co., Ltd., Tokyo, Japan). The intracranial volume (ICV) was manually traced based on Whitewell et al [ref 22]. This manual tracing was preceded in axial view from superior to inferior, by drawing around the dura, excluding any bone marrow or any non-brain but including the superior sagittal sinus. Optic nerves were also excluded. The whole brain volume was measured based on Matsuzawa et al [ref 9]. Total brain volume (TBV) was defined by the brain region without CSF (Cerebrospinal fluid), which was masked with similar procedures for ICV using Dr. View. The caudal brain stem regions below the level of the cerebral peduncles of the midbrain were excluded. The hypothalamic and chiasmatic cisternae were retained, but the pituitary, the carotid cisterna, the ambient cisterna and the quadrigeminal plates were excluded." |