| Range |
~0.02 %
|
| Organism |
Generic |
| Reference |
Sasaki N, Gunji Y, Kase C, Sato K. Molecular crowding improves bead-based padlock rolling circle amplification. Anal Biochem. 2016 Dec 7 519: 15-18. doi: 10.1016/j.ab.2016.12.002. p.15 left column top paragraphPubMed ID27940012
|
| Primary Source |
[1] Jarvius J et al., Digital quantification using amplified single-molecule detection. Nat Methods. 2006 Sep3(9):725-7.PubMed ID16929318
|
| Comments |
P.15 left column top paragraph: "The ultrasensitive and accurate detection of DNA from biological samples is important in various strategies related to medical diagnosis. For example, pathogenic bacteria from a patient can be identified by DNA sequences specific to the bacteria, and suitable pharmaceutical treatment strategies can then be employed. The typing of single nucleotide polymorphism (SNP) or copy number variation (CNV) is also useful in estimating the effectiveness of a drug and the risk of future diseases. Padlock rolling circle amplification (RCA) is a promising technique to achieve these ends [primary source]. In padlock RCA, fluorescent microdots are produced in the presence of a target DNA sequence. However, the detection efficiency (the number of detected fluorescent microdots per estimated number of products) was reported to be ∼0.02% [primary source], which is far from efficient detection system." See BNID 113084 |
| Entered by |
Uri M |
| ID |
113083 |