| Range |
0.1–0.4 µm^2/sec
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| Organism |
Mouse Mus musculus |
| Reference |
Yan X, Hoek TA, Vale RD, Tanenbaum ME. Dynamics of Translation of Single mRNA Molecules In Vivo. Cell. 2016 May 5 165(4):976-89. doi: 10.1016/j.cell.2016.04.034. p.978 right column top paragraphPubMed ID27153498
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| Primary Source |
Katz ZB et al., Mapping translation 'hot-spots' in live cells by tracking single molecules of mRNA and ribosomes. Elife. 2016 Jan 135. pii: e10415. doi: 10.7554/eLife.10415.PubMed ID26760529
|
| Method |
Primary source abstract: "Therefore, [investigators] imaged single β-actin mRNAs tagged with MS2 stem loops colocalizing with labeled ribosomes to determine when polysomes formed." Primary source p.13 2nd paragraph: "Mouse embryonic fibroblasts (MEFs) derived from the MS2 β-actin knock-in mouse (Lionnet et al., 2011) were used for tracking endogenous β-actin mRNA as described previously." |
| Comments |
P.978 left column bottom paragraph: "When observed by spinning disk confocal microscopy, the co-expression of a reporter construct (SunTag24x-Kif18b-PP724x, with Kif18b being a kinesin motor with a 2.5 kb coding sequence Tanenbaum et al., 2011), scFv-GFP and PP7-mCherry, resulted in the appearance of a small number (10–50) of very bright green and red fluorescent spots per cell that co-migrated in time-lapse movies (Figure 1C Movie S1). Spot tracking revealed that these spots diffused with a diffusion coefficient of 0.047 μm^2/s, which is slightly slower than previous measurements of mRNA diffusion (0.1–0.4 μm^2/s) (primary source), consistent with the fact that [investigators’] reporter mRNA contains a larger open reading frame (4.4 kb versus 1.1 kb) and thus more associated ribosomes." |
| Entered by |
Uri M |
| ID |
112741 |