| Range |
corralled diffusion 0.02µm^2/sec: random diffusion 0.09µm^2/sec µm^2/sec
|
| Organism |
Fruit fly Drosophila melanogaster |
| Reference |
Halstead JM et al., An RNA biosensor for imaging the first round of translation from single cells to living animals. Science. 2015 Mar 20 347(6228):1367-671. doi: 10.1126/science.aaa3380. p.1368 middle columnPubMed ID25792328
|
| Method |
Abstract:"[Investigators] have developed a fluorescence microscopy technique that reports on the first translation events of individual mRNA molecules." Single-particle tracking (SPT) |
| Comments |
P.1368 middle column:"Although translation is thought to occur exclusively in the cytoplasm, recent studies suggest that protein synthesis can occur in the nucleus (refs 9, 10). Because the TRICK [translating RNA imaging by coat protein knock-off] assay can distinguish between untranslated and translated mRNAs, [investigators] imaged TRICK reporter mRNAs in the nucleus 30 min after ponA [ponasterone A ] induction. Single-particle tracking (SPT) of nuclear mRNAs determined that they undergo both corralled (D = 0.02 μm^2/s) and random diffusion (D = 0.09 μm^2/s), similar to the movements observed for other nuclear mRNAs (refs 11, 12)." |
| Entered by |
Uri M |
| ID |
112164 |