Number of all plasma membrane transporters & other integral membrane proteins in HeLa cell

Range all plasma membrane transporters 4×10^7copies: other integral membrane proteins ~3×10^7copies protein copies
Organism Human Homo sapiens
Reference Itzhak DN, Tyanova S, Cox J, Borner GH. Global, quantitative and dynamic mapping of protein subcellular localization. Elife. 2016 Jun 9 5. pii: e16950. doi: 10.7554/eLife.16950. p.9 top paragraphPubMed ID27278775
Method Abstract: "[Investigators] have developed and applied Dynamic Organellar Maps, a proteomic method that allows global mapping of protein translocation events. [They] initially used maps statically to generate a database with localization and absolute copy number information for over 8,700 proteins from HeLa cells, approaching comprehensive coverage. All major organelles were resolved, with exceptional prediction accuracy (estimated at >92%). Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. [They] subsequently demonstrated the dynamic capabilities of the approach by capturing translocation events following EGF [epidermal growth factor] stimulation, which [they] integrated into a quantitative model."
Comments P.9 top paragraph: "This predominance probably reflects the adaptation of HeLa cells for fast nutrient uptake to support rapid growth. Supporting this view, all plasma membrane transporters combined (40 million copies) contribute approximately 25% of the total compartment protein mass. Other integral membrane proteins (such as adhesion and signalling receptors) contribute ∼30 million copies."
Entered by Uri M
ID 112706