Level of expression of most protein coding genes in lymphoblastoid cell line GM12878

Range 1 - 50 protein copies/cell
Organism Human Homo sapiens
Reference Marinov GK et al., From single-cell to cell-pool transcriptomes: stochasticity in gene expression and RNA splicing. Genome Res. 2014 Mar24(3):496-510. doi: 10.1101/gr.161034.113 p.501 right column 2nd paragraphPubMed ID24299736
Method Abstract:"Here [investigators] apply the SMART-seq single-cell RNA-seq protocol to study the reference lymphoblastoid cell line GM12878."
Comments p.501 right column 2nd paragraph:”[Investigators] use the transcripts-per-cell estimates for all subsequent analyses. Previous studies have reported that genes can be separated into two distinct groups based on their expression levels—one group expressed at high (>1 FPKM) levels and one at very low (<<1 FPKM) levels (Hebenstreit et al. 2011). [They] examined the distribution of estimated copies per cell in single cells and in pool/splits (Fig. 3A). [They] find that in individual cells, most protein coding genes are expressed at levels between 1 and ~50 copies per cell. The distribution suggests a roughly equal number of genes at each level except for a larger group of transcripts with fractional transcript-per-cell values.” FPKM=substitute reads with fragments
Entered by Uri M
ID 111778