| Comments |
P.4 left column bottom paragraph: "Conservation of absolute metabolite concentrations: Given the fundamental connection between ΔG and metabolite concentrations, the similarity of reaction free energies across species suggested similarity in the metabolomes. A compilation of metabolite concentrations, determined by a combination of direct concentration measurements and the above approach for integrating free energy measurements, is provided in Supplementary Table 5." P.5 right column bottom paragraph: "In addition to the methodology for measuring ΔG, a key contribution of this work is a coherent systems-level set of fluxes, free energies and metabolite concentrations for E. coli, yeast and the mammalian cell line iBMK (Figs. 2–5 and Supplementary Tables 1, 4 and 5)." See notes beneath table. iBMK=Immortalized Baby Mouse Kidney Epithelial Cells |