| Range |
∼1 vesicle/sec
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Kuromi, H., & Kidokoro, Y. (2003). Two synaptic vesicle pools, vesicle recruitment and replenishment of
pools at the Drosophila neuromuscular junction. Journal of neurocytology, 32(5-8), 551-565. p.558 right column 4th paragraphPubMed ID15034253
|
| Primary Source |
Sankaranarayanan S, Ryan TA. Calcium accelerates endocytosis of vSNAREs at hippocampal synapses. Nat Neurosci. 2001 Feb4(2):129-36.PubMed ID11175872
|
| Method |
Primary source abstract: "A pH-sensitive form of green-fluorescent protein (GFP) fused to the lumenal domain of VAMP (synapto-pHluorin) provides a sensitive optical probe to track the net balance between exocytosis and endocytosis of this protein at small synaptic terminals of the central nervous system. Here [investigators] used a reversible proton-pump blocker that prevents vesicle re-acidification upon endocytosis to trap vesicles in the alkaline state during recycling. In combination with optical measurements of synapto-pHluorin, [they] used alkaline trapping to examine the kinetic components of exocytosis and endocytosis separately at synaptic terminals." |
| Comments |
P.558 right column 4th paragraph: "At room temperature in shibire and wild-type larvae, the recycling of SVs [synaptic vesicles] maintains synaptic transmission during tetanic stimulation. The maximum recycling rate was ∼1000/s at this synapse, which corresponds to ∼2 vesicles/release site/s (number of release sites,
550, Atwood et al., 1993). This value is similar to that estimated for the maximum endocytosis rate, ∼1 vesicle/s, at rat hippocampal synapses in culture (primary source)." |
| Entered by |
Uri M |
| ID |
112563 |