Movement rate of FM [fluorescent membrane] 1-43 presynaptic puncta in culture

Value 1.96 µm/min Range: ±0.25 µm/min
Organism Rat Rattus norvegicus
Reference Hopf FW, Waters J, Mehta S, Smith SJ. Stability and plasticity of developing synapses in hippocampal neuronal cultures. J Neurosci. 2002 Feb 1 22(3):775-81. p.776 left column bottom paragraphPubMed ID11826107
Method p.776 left column 4th paragraph: "Dissociated cultures were prepared from embryonic rat hippocampi using the methods of Banker and Goslin (1996). [Investigators] measured presynaptic vesicle turnover at visually distinct puncta using established FM 1–43 imaging methods (Ryan et al., 1996). Platinum field electrodes were used to evoke action-potential firing to load and unload FM 1–43. In all experiments, dye loading was induced by 100 stimuli with dye in the extracellular solution. Cells were then washed to remove extracellular dye, and images were acquired. FM 1–43 was released with 600 stimuli, and images of background (nonreleasable) staining were acquired."
Comments p.776 left column bottom paragraph:"In the course of [investigators'] experiments, [they] observed a population of mobile FM 1–43-loaded puncta. These puncta moved at a rate of 1.96 ± 0.25 μm/min (determined for 18 puncta), consistent with recent reports that describe mobile, nonsynaptic clusters of vesicles that load and unload FM 1–43 during stimulation (Kraszewski et al., 1995 Dai and Peng, 1996)."
Entered by Uri M
ID 112036