| Comments |
P.625 2nd paragraph:"Effect of heat pretreatment on inactivation of D-glucose flux in ghosts: It is a well known fact that enzyme activities or biological phenomena in general show a well defined heat inactivation [ref 26]. In order to study this aspect of the temperature effect on this system, the ghosts were pretreated for 60 min at a high temperature, up to 80°, and the fluxes of glucose and mannitol were measured at these same temperatures (Table IV). The following points are notable. The observed glucose flux at the higher sugar concentration (130.4mM) was increased considerably as the temperature was raised from 37 to 65°. The flux at the lower sugar concentration (5mM), on the other hand, showed a broad optimum at around 45°, with the flux at 65° being less than the maximum by only 30% and still greater than the flux at 23°." See note above table. Red cell ghost="In a blood smear, a large, pale, crescent-shaped cell devoid of internal contents and produced by hemolysis. Such cells are always indicative of disease." [The free dictionary] |