Time to transport the 169-kbp genome into the cell cytoplasm

Value 30 sec
Organism Bacteriophage T4
Reference Choi KH et al., Insight into DNA and protein transport in double-stranded DNA viruses: the structure of bacteriophage N4. J Mol Biol. 2008 May 2 378(3):726-36. doi: 10.1016/j.jmb.2008.02.059. p.726 right columnPubMed ID18374942
Primary Source Boulanger, P. & Letellier, L. (1988). Characterization of ion channels involved in the penetration of phage T4 DNA into Escherichia coli cells. J. Biol. Chem. 263, 9767–9775.PubMed ID2454920
Method Primary source abstract: "[Investigators] have analyzed the kinetics of the K+ efflux induced by T4 phage and ghosts (phage depleted of DNA) using a potassium selective electrode."
Comments P.726 right column: "T4, for example, attaches to the cell surface via fibers that are attached to a baseplate at the tip of the tail. Upon successful adsorption, conformational changes in the baseplate trigger contraction of a tail sheath, which then drives the tail tube through the cell envelope [ref 1]. T4 transports a 169-kbp genome into the cell cytoplasm in 30 s. This is one of the most efficient DNA transport processes known [primary source]."
Entered by Uri M
ID 112413