| Range |
average 25nts/sec: measured at trp operon 27nts/sec: estimated at bulk mRNA 29nts/sec nts/sec
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Chen H, Shiroguchi K, Ge H, Xie XS. Genome-wide study of mRNA degradation and transcript elongation in Escherichia coli. Mol Syst Biol. 2015 Jan 12 11(1):781. doi: 10.15252/msb.20145794. p.4 right column 2nd paragraphPubMed ID25583150
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| Primary Source |
Manor H, Goodman D, Stent GS (1969) RNA chain growth rates in Escherichia coli. J Mol Biol 39: 1–29 & Rose JK, Mosteller RD, Yanofsky C (1970) Tryptophan messenger ribonucleic acid elongation rates and steady‐state levels of tryptophan operon enzymes under various growth conditions. J Mol Biol 51: 541–550PubMed ID4938814, 4923859
|
| Method |
Abstract:"Here, [investigators] report a genome-wide RNA degradation study in Escherichia coli using RNA-seq, and present evidence that the stereotypical exponential RNA decay curve obtained using initiation inhibitor, rifampicin, consists of two phases: residual RNA synthesis, a delay in the interruption of steady state that is dependent on distance relative to the mRNA's 5' end, and the exponential decay." |
| Comments |
P.4 right column 2nd paragraph:"[Investigators] were able to measure RNA chain elongation rates for 263 transcripts of > 1,200 nt length that have enough data for analysis, producing a survey of native mRNA chain elongation rates (Fig 3B Supplementary Table S6). [Their] average elongation rate of 25 nt/s agrees with previous measurements of native mRNA transcription elongation rates. The trp operon was previously measured at 27 nt/s, and bulk mRNA elongation rates were estimated at 29 nt/s (primary sources)." |
| Entered by |
Uri M |
| ID |
112325 |