Average mutation rate

Range transitions 2.5×10^-5 to 2.6×10^-4: transversions 1.2×10^-6 to 1.5×10^-5 substitutions per site
Organism Poliovirus
Reference Acevedo A, Brodsky L, Andino R. Mutational and fitness landscapes of an RNA virus revealed through population sequencing. Nature. 2014 Jan 30 505(7485):686-90. doi: 10.1038/nature12861. p.687 right column top paragraphPubMed ID24284629
Method P.686 left column 2nd paragraph:"To overcome the limitations of next-generation sequencing error, [investigators] developed circular sequencing (CirSeq), wherein circularized genomic RNA fragments are used to generate tandem repeats that then serve as substrates for next-generation sequencing (for DNA adaptation, see ref. 4). The physical linkage of the repeats, generated by ‘rolling circle’ reverse transcription of the circular RNA template, provides sequence redundancy for a genomic fragment derived from a single individual within the virus population (Fig. 1a and Extended Data Fig. 1)."
Comments P.687 left column:"[Investigators] find that mutation rates vary by more than two orders of magnitude depending on mutation type, transitions averaging 2.5 × 10^−5 to 2.6 × 10^−4 substitutions per site and transversions averaging 1.2 × 10^−6 to 1.5 × 10^−5 substitutions per site (Fig. 3)."
Entered by Uri M
ID 112261