Total concentration of actin and related proteins

Range G-actin 100μM: profilin 10μM: thymosin-β4 200 μM μM
Organism Unspecified
Reference Kiuchi T, Nagai T, Ohashi K, Mizuno K. Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension. J Cell Biol. 2011 Apr 18 193(2):365-80. doi: 10.1083/jcb.201101035. p.368 right column bottom paragraphPubMed ID21502360
Primary Source Mogilner, A., and L. Edelstein-Keshet. 2002. Regulation of actin dynamics in rapidly moving cells: a quantitative analysis. Biophys. J. 83: 1237–1258. doi:10.1016/S0006-3495(02)73897-6PubMed ID12202352
Comments P.368 right column bottom paragraph to p.370 left column top paragraph:"Assuming that the total concentrations of G-actin, profilin, and thymosin-β4 are 100, 10, and 200 μM (primary source), respectively, the cellular concentrations of free G-actin, profilin- and thymosin-β4–bound G-actin, and free barbed and pointed ends of actin filaments under unstimulated conditions were estimated as 1.98 μM, 9.52 μM, 88.5 μM, 4.8 nM, and 5.5 nM, respectively, from the best-fit of the time curve of relative FDAP (fluorescence decay after photoactivation, see Materials and methods). Thus, the high time resolution and quantitative features of s-FDAP analysis allowed [investigators] to estimate the cellular concentrations of key components involved in the regulation of actin dynamics by model analysis."
Entered by Uri M
ID 112134