Doubling time when grown at 35°C in minimal media

Value 120 min Range: ±2 min
Organism Bacteria Bacillus subtilis
Reference I.D.J.Burdett et. al. Growth kinetics of individual Bacillus subtilis cells and correlation with nucleoid extension. J Bacteriol. 1986 Jul 167(1): 219–230. abstract & p.221 right column 2nd paragraphPubMed ID3087953
Method Abstract:"The growth rate of individual cells of Bacillus subtilis (doubling time, 120 min) has been calculated by using a modification of the Collins-Richmond principle which allows the growth rate of mononucleate, binucleate, and septate cells to be calculated separately. The standard Collins-Richmond equation represents a weighted average of the growth rate calculated from these three major classes." P.219 right column 4th paragraph: "B. subtilis 168/S (refs 4, 46, 47), which is able to grow on succinate as a sole carbon source, was grown at 35°C in minimal medium (ref 47) with 0.5% glucose for six generations and then transferred to medium containing 0.4% sodium succinate. Cultures were inoculated from a single colony and periodically subcultured over 3 days to maintain an absorbancy at 600 nm of <0.3."
Comments P.221 right column 2nd paragraph: "The doubling time (TD) of the culture, as measured by the increase in absorbancy at 600 nm, was 120.08 ± 2.16 min (mean ± standard deviation). The specific growth rate (k) was 0.0058 ± 0.0001 doubling times per min." Please note-this value is for minimal medium (see Method section). For shorter doubling times see BNID 111071
Entered by Slava
ID 112127