Synthesis and degradation rate constant of C4-HSL (C4-homo-serine lactone)

Range synthesis rate 48,000nM/hr: degradation rate 0.0133/hour
Organism Bacteria Pseudomonas aeruginosa
Reference Pai A, You L: Optimal tuning of bacterial sensing potential. Molecular systems biology 2009, 5(286) :286 doi: 10.1038/msb.2009.43. Supplementary Text 2 p.27 bottom paragraphPubMed ID19584835
Primary Source Glansdorp FG et al., (2004) Synthesis and stability of small molecule probes for Pseudomonas aeruginosa quorum sensing modulation. Organic & biomolecular chemistry 2: 3329-3336. AND Parsek MR, Val DL, Hanzelka BL, Cronan JE, Jr., Greenberg EP (1999) Acyl homoserine-lactone quorum-sensing signal generation. Proceedings of the National Academy of Sciences of the United States of America 96: 4360-4365.PubMed ID15534711, 10200267
Comments Supplementary Text 2 p.27 bottom paragraph:"In the rhl module (primary source Parsek et al., 1999) the signal C4-HSL, synthesized by RhlI, controls the production of rhamnolipid, a surfactant toxin (Ochsner & Reiser, 1995). Synthesis rate: The turnover rate (primary source Parsek et al., 1999) of the RhlI is 16 mol C4-HSL mol^-1 of RhlI min^-1, giving k=16×60×50=4.8×10^4 nM/hr. Degradation rate constant: The half life of C4-HSL at physiological temperatures is reported as 2 days (primary source Glansdorp et al., 2004) giving da=0.0133hr^-1."
Entered by Uri M
ID 112008