Three-dimensional profiles of cells

Range Table - link
Organism Mycobacterium tuberculosis
Reference Yamada H, Yamaguchi M, Chikamatsu K, Aono A, Mitarai S. Structome analysis of virulent Mycobacterium tuberculosis, which survives with only 700 ribosomes per 0.1 fl of cytoplasm. PLoS One. 2015 Jan 28 10(1):e0117109. doi: 10.1371/journal.pone.0117109. p.7/14 table 3PubMed ID25629354
Method p.3 bottom paragraph:"Images obtained from negative scanning through Adobe Photoshop Elements (version 9) with CanoScan 8800F were saved as TIFF files and analyzed using ImageJ and Fiji software [refs 15, 16]. Briefly, cell length was calculated by multiplying the number by 55 nm (representing the thickness of each section). The diameter (minor and major axes), perimeter, and thickness of the plasma membrane (PM), outer membrane (OM), and cell envelope of each cell were measured as a pixel value using the line selection menu in the ImageJ/Fiji window as well as a scale bar recorded on the same negative. Measured pixel values were converted to μm or nm according to the measured pixel value of the scale bar on the corresponding negatives."
Comments p.7 2nd paragraph:"Three-dimensional (3D) analysis-The volume of each of the five M. tuberculosis cells was calculated using measurements taken with ImageJ/Fiji software, as described in the Materials and Methods section. Data regarding the 3D profile of each cell are shown in Table 3 and Fig. 3. The average whole-cell (as outlined by the OM) and cytoplasmic (as outlined by the PM) volumes were 0.293 ± 0.113 fl (range, 0.177–0.429 fl) and 0.210 ± 0.091 fl (range, 0.110–0.306 fl), respectively. In addition, the average OM, PM, and periplasmic volumes were 0.006 ± 0.003 fl (range, 0.002–0.009 fl), 0.019 ± 0.008 fl (range, 0.008–0.027 fl), and 0.060 ± 0.021 fl (range, 0.031–0.088 fl), respectively. Because the volumes of cells 3, 4, and 5 were 2-fold greater than that of the smallest cell (cell 1), it is suggested that cell division may occur independently of the volume of the parent cell."
Entered by Uri M
ID 111988