One-dimensional profiles of cells

Range Table - link
Organism Mycobacterium tuberculosis
Reference Yamada H, Yamaguchi M, Chikamatsu K, Aono A, Mitarai S. Structome analysis of virulent Mycobacterium tuberculosis, which survives with only 700 ribosomes per 0.1 fl of cytoplasm. PLoS One. 2015 Jan 28 10(1):e0117109. doi: 10.1371/journal.pone.0117109. p.5/14 table 1PubMed ID25629354
Method p.3 bottom paragraph:"Images obtained from negative scanning through Adobe Photoshop Elements (version 9) with CanoScan 8800F were saved as TIFF files and analyzed using ImageJ and Fiji software [refs 15, 16]. Briefly, cell length was calculated by multiplying the number by 55 nm (representing the thickness of each section). The diameter (minor and major axes), perimeter, and thickness of the plasma membrane (PM), outer membrane (OM), and cell envelope of each cell were measured as a pixel value using the line selection menu in the ImageJ/Fiji window as well as a scale bar recorded on the same negative. Measured pixel values were converted to μm or nm according to the measured pixel value of the scale bar on the corresponding negatives."
Comments p.4 bottom paragraph:"The length and diameter of five M. tuberculosis cells were measured using ImageJ/Fiji software, as described in the Materials and Methods section. Table 1 lists the results of a one-dimensional structome analysis of the five cells." P.5 bottom paragraph:"Cell diameter values were very similar, with average OM [outer membrane] and PM [plasma membrane] diameters of 0.345 ± 0.029μm (range, 0.293–0.366 μm) and 0.297 ± 0.022 μm (range, 0.273–0.326 μm), respectively. The average aspect ratio was 8.23 ± 3.60 (Table 1 and Fig. 2). Because the lengths of cells 3, 4, and 5 were 3-fold greater than that of the smallest cell (cell 1), it is suggested that cell division may occur independently of the length of the parent cell."
Entered by Uri M
ID 111986