Relative contribution of metabolic (by) products to the carbon balance of C-, N-, or P-limited chemostat cultures at dilution rates of 0.1 and 0.4 h^−1

Range Table - link % of carbon recovered
Organism Bacteria Bacillus subtilis
Reference Dauner M, Storni T, Sauer U (2001) Bacillus subtilis metabolism and energetics in carbon-limited and excess-carbon chemostat culture. J Bacteriol 183: 7308–7317 p.7311 table 1PubMed ID11717290
Primary Source See ref beneath table
Method p.7309 left column 2nd paragraph:"The primary focus of this work was to quantify the magnitude of energy dissipation via overflow metabolism, metabolic shifts, and futile enzyme cycling in C-limited and excess-C Bacillus subtilis chemostat cultures, by using isotopomer-balancing (ref 9) and [U-13C6]glucose-labeling experiments (ref 55). Thus, for the first time [investigators] report here intracellular carbon flux distributions in N- and P-limited B. subtilis."
Comments p.7311 left column bottom paragraph:"Formation of low-molecular-weight by-products (excluding riboflavin) by so-called overflow metabolism was low in C-limited culture (ref 10 beneath table), moderate in N-limited culture, and extensive in P-limited culture (Table 1), as was described for Bacillus spp. (refs 28, 35) and E. coli (ref 31). The primary by-product was acetate, but diacyl and acetoin formation was also significant. The specific production rate of acetate was strongly influenced by the growth rate and was highest under P limitation (Fig. 3). Not generally considered classical products of overflow metabolism, extracellular protein and cell wall components contributed 1 to 4% of the carbon balance (data not shown). Recombinant riboflavin production contributed very little to the carbon balance and showed no pronounced response to the different environmental conditions (Table 1)."
Entered by Uri M
ID 111948