| Value |
0.4
mRNAs/gene/cell
Range: 0.02 - 3 mRNAs/gene/cell
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Moran MA et al., Sizing up metatranscriptomics. ISME J. 2013 Feb7(2):237-43. doi: 10.1038/ismej.2012.94. p.237 left column top paragraphPubMed ID22931831
|
| Primary Source |
Taniguchi Y, Choi PJ, Li GW, Chen H, Babu M, Hearn J, Emili A, Xie XS. Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science. 2010 Jul 30 329(5991):533-8. Supplementary Table S6PubMed ID20671182
|
| Method |
High-resolution fluorescence detection of tagged mRNAs |
| Comments |
p.237 left column top paragraph:"Classic microbiological studies of the composition of exponentially growing Escherichia coli concluded that each cell harbors ~1380 mRNA molecules (Neidhardt, 1996), a small number compared with other macromolecule inventories (that is, >3000 genes and >2 000 000 proteins). Similarly, recent single-molecule detection in individual E. coli cells based on high-resolution fluorescence detection of tagged mRNAs determined that the number of transcripts per gene per cell averaged only 0.4 (range: 0.02–3 see Supplementary Table S6 in primary source)." |
| Entered by |
Uri M |
| ID |
111919 |