Number of mRNA transcripts per cell

Value 26000 mRNAs/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Pelechano V, Chávez S, Pérez-Ortín JE. A complete set of nascent transcription rates for yeast genes. PLoS One. 2010 Nov 16 5(11):e15442. p.2 left column bottom paragraphPubMed ID21103382
Primary Source Miura F, Kawaguchi N, Yoshida M, Uematsu C, Kito K, Sakaki Y, Ito T. Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs. BMC Genomics. 2008 Nov 29 9 :574 AND Nagalakshmi U, Wang Z, Waern K, Shou C, Raha D, Gerstein M, Snyder M. The transcriptional landscape of the yeast genome defined by RNA sequencing. Science. 2008 Jun 6 320(5881):1344-9.PubMed ID19040753, 18451266
Method (1st primary source abstract:)"[Researchers] conceived an idea of competitive PCR between genomic DNA and cDNA." (2nd primary source abstract:) "[Researchers] developed a quantitative sequencing-based method called RNA-Seq for mapping transcribed regions, in which complementary DNA fragments are subjected to high-throughput sequencing and mapped to the genome."
Comments "[Researchers] used an updated total amount of mRNA molecules per yeast cell that has been corrected from that previously used (15000 molecules from [Hereford et al., 1977 PMID 321129]) to more recent and precise calculations obtained from massive parallel sequencing and competitive PCR transcriptome measurements: 26000 molecules from [primary sources]." See BNID 102988, 103023
Entered by Uri M
ID 106763