| Range |
≤10 µm^2/sec
|
| Organism |
Mammals |
| Reference |
Veith R et al., Balbiani ring mRNPs diffuse through and bind to clusters of large intranuclear molecular structures. Biophys J. 2010 Oct 20 99(8):2676-85. doi: 10.1016/j.bpj.2010.08.004. p.2676 left column bottom paragraphPubMed ID20959109
|
| Primary Source |
[13] Politz J.C., Browne E.S., Pederson T. Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells. Proc. Natl. Acad. Sci. USA. 1998 95: 6043–6048. [14] Politz J.C., Tuft R.A., Singer R.H. Movement of nuclear poly(A) RNA throughout the interchromatin space in living cells. Curr. Biol. 1999 9 :285–291.PubMed ID9600914, 10209094
|
| Method |
Fluorescence Correlation Spectroscopy (FCS) |
| Comments |
p.2676 left column bottom paragraph:"In mammalian cells, the interphase nucleus is crowded with both hetero- and euchromatin, affecting the mobility of every macromolecule or macromolecular complex, and of course also that of mRNPs (ref 21). Using the almost pointlike observation volume of a fluorescence correlation spectroscopy (FCS) instrument, Politz et al. (primary source 13) showed that mRNP particles can move in cell nuclei almost as fast as in aqueous solution, with diffusion coefficients up to 10 µm^2/s (primary sources 13,14). These researchers argued that such fast diffusion could occur within interchromatin channels (ref 22)." Please note-this range [=10µm^2/sec] wasn't located in primary sources |
| Entered by |
Uri M |
| ID |
111856 |