Density of labeled messenger ribonucleoprotein particles [mRNPs] in nucleus

Range ≈10 mRNPs/µm^3
Organism Midge Chironomus tentans
Reference Siebrasse JP et al., Discontinuous movement of mRNP particles in nucleoplasmic regions devoid of chromatin. Proc Natl Acad Sci U S A. 2008 Dec 23 105(51):20291-6. doi: 10.1073/pnas.0810692105. p.20292 right column top paragraphPubMed ID19074261
Method p.20291 right column 3rd paragraph:"In the present study, [investigators] have analyzed the intranuclear mobility of a single native mRNP species, the BR2 [Balbiani Ring 2] mRNP. Fluorescence labeling was achieved in vivo by microinjection of fluorescent oligonucleotides (DNA or RNA), which were complementary to a highly repetitive coding sequence in BR2 mRNA."
Comments p.20292 right column top paragraph:"Having shown that BR2 mRNPs can be specifically labeled in vivo, [investigators] next optimized the visualization and tracking of single particles in the nucleoplasm by using a high-speed laser scanning microscope (LSM). This line-scanning LSM yielded high signal-to-noise ratios (SNR) and frame rates. However, because of the high density of labeled mRNPs (approximately 10 per µm^3), a short photobleaching phase had to be included to singularize particles for tracking."
Entered by Uri M
ID 111857