| Comments |
p.604 left column bottom paragraph:"[Investigators] generated a stable cell line, derived from a transgenic mouse, where all ß-actin mRNA is labelled by yellow fluorescent protein (YFP) fused to a MS2 protein tag [ref 9, 10] (Methods and Supplementary Figs 1 and 2). ß-Actin is an essential gene and the mRNA transcript has an estimated size of less than 25 nm, being diffraction limited even if the MS2 sequence should be extended (Supplementary Information)." Immortalized mouse embryo fibroblast cells (MEFs) |