Average rate at which B cells divide

Range young adults 1.9±1.0: elderly 1.5±1.3 % of total B cells/day
Organism Human Homo sapiens
Reference Macallan DC et al., B-cell kinetics in humans: rapid turnover of peripheral blood memory cells. Blood. 2005 May 1 105(9):3633-40. abstract, p.3635 left column 3rd paragraph & p.3638 left column 2nd paragraphPubMed ID15644412
Method p.3634 left column 5th paragraph:"Proliferation and disappearance rates of lymphocytes in vivo were measured by labeling dividing cells in vivo with deuterated glucose. Labeling consisted of a primed 24-hour intravenous infusion of 1g/kg 6,6-2H2-glucose (Cambridge Isotopes, MA), during which diet was restricted. Blood for measurement of plasma glucose deuterium (2H) enrichment was taken after 1 hour and approximately every 4 hours thereafter during the infusion. Blood samples for estimation of deuterium enrichment in DNA of B lymphocytes were taken 3, 4, 10, and 21 days after infusion, or on the closest days possible. Day-3 samples were omitted in the first 3 controls and the elderly cohort in the latter, to minimize blood sampling."
Comments p.3635 left column 3rd paragraph:"Following infusion of 6,6 2H2-glucose, enrichment of deuterium in DNA from B cells was found in most young subjects to peak at day 3 or 4 and fall thereafter (Figure 1). Best fit curves based on the model described in the previous section were plotted (Figure 1), and values for proliferation and disappearance rate constants were derived (Table 1). Mean B-cell proliferation rate was 1.9% (±1.0%) per day, equivalent to a doubling time of cells within this population of about 36 days (Table 1). Disappearance rates exceeded proliferation rates for most subjects, although for CO4 and CO7 no disappearance rate could be modeled, and it was taken as zero. Mean disappearance rate for the whole group (n = 8) was 3.9% (±3.4%) per day (Table 1), equivalent to a half-life of about 18 days."
Entered by Uri M
ID 111626