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Cytosolic pH of Gef1 expressing cell

Range	~6 unitless
Organism	Budding yeast Saccharomyces cerevisiae
Reference	Braun NA, Morgan B, Dick TP, Schwappach B. The yeast CLC protein counteracts vesicular acidification during iron starvation. J Cell Sci. 2010 Jul 1 123(Pt 13):2342-50. doi: 10.1242/jcs.068403. p.2345 right column bottom paragraphPubMed ID20530571
Method	"Steady-state pH measurements of the cytosol, the Gef1- containing compartment and the vacuole were then performed with gef1 deletion strains expressing the different Gef1 variants (Fig. 5)."
Comments	"By homology, the only CLC protein of Saccharomyces cerevisiae, Gef1, belongs to this family of intracellular exchangers." "Expression of any Gef1 variant resulted in a cytosolic pH close to 6 (differences to the deletion strain are statistically significant with P <0.02). At the same time, the Gef1-containing compartment was slightly acidic with respect to the cytosol and the vacuolar lumen substantially more acidic (Fig. 5B,C,D,E), as expected for these acidifying compartments from the literature (Klionsky et al., 1992, Manolson et al., 1994, Martinez-Munoz and Kane, 2008, Plant et al., 1999, Rothman et al., 1989)."
Entered by	Uri M
ID	111466