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||Budding yeast Saccharomyces cerevisiae
||Valli M et al., Intracellular pH distribution in Saccharomyces cerevisiae cell populations, analyzed by flow cytometry. Appl Environ Microbiol. 2005 Mar71(3):1515-21. doi: 10.1128/AEM.71.3.1515-1521.2005. p.1518 figure 4PubMed ID15746355
||"The cells were harvested in exponential (¦) and stationary phase (?), loaded with the fluorochrome, and incubated in McIlvaine buffers of
different pH values. The mean values of pHi obtained from two independent
experiments are plotted versus the external pH. Standard deviations are indicated."
||"Figure 4 shows the mean values of two independent experiments.
Interestingly, the cells derived from different growth
phases behave significantly differently under the chosen condition
at different pH values. By decreasing the external pH from 7.0 to 2.2, a progressive reduction of the pHi from 7.1 to
5.1 was observed in exponentially grown cells. In contrast,
stationary cells, which were able to maintain the pHi constant
at around 6.1 when the external pH was in the range of 7.0 to
5.5, experienced a drop in pHi to 5.5 as a consequence of a
reduction of the external pH to 5.0. Further reductions in the
external pH did not have any effect. In fact a pHi of 5.5 was
maintained by the stationary cells even with decreasing pH
values from 5.0 to 2.2."