Effect of the external pH on pHi [intracellular pH] of cells

Range Figure - link unitless
Organism Budding yeast Saccharomyces cerevisiae
Reference Valli M et al., Intracellular pH distribution in Saccharomyces cerevisiae cell populations, analyzed by flow cytometry. Appl Environ Microbiol. 2005 Mar71(3):1515-21. doi: 10.1128/AEM.71.3.1515-1521.2005. p.1518 figure 4PubMed ID15746355
Method "The cells were harvested in exponential (¦) and stationary phase (?), loaded with the fluorochrome, and incubated in McIlvaine buffers of different pH values. The mean values of pHi obtained from two independent experiments are plotted versus the external pH. Standard deviations are indicated."
Comments "Figure 4 shows the mean values of two independent experiments. Interestingly, the cells derived from different growth phases behave significantly differently under the chosen condition at different pH values. By decreasing the external pH from 7.0 to 2.2, a progressive reduction of the pHi from 7.1 to 5.1 was observed in exponentially grown cells. In contrast, stationary cells, which were able to maintain the pHi constant at around 6.1 when the external pH was in the range of 7.0 to 5.5, experienced a drop in pHi to 5.5 as a consequence of a reduction of the external pH to 5.0. Further reductions in the external pH did not have any effect. In fact a pHi of 5.5 was maintained by the stationary cells even with decreasing pH values from 5.0 to 2.2."
Entered by Uri M
ID 110912