| Range |
with constant illumination 5min: with flash illumination 17min min
|
| Organism |
Mouse Mus musculus |
| Reference |
Saari JC. Biochemistry of visual pigment regeneration: the Friedenwald lecture. Invest Ophthalmol Vis Sci. 2000 Feb41(2):337-48. p.341 left column top paragraphPubMed ID10670460
|
| Method |
"Flash illumination of animals and humans has been used
with great success in a number of experimental situations.
However, it could be argued that physiological conditions are
more closely approximated with steady illumination. Thus, [researchers]
thought it important to verify that [their] observation of the
accumulation of all-trans-retinal during recovery from a flash
was not an artifact of the illumination conditions. Dark-adapted
mice were subjected to illumination from two 60-W fluorescent
bulbs (50 foot-candles). Retinoids were extracted and
analyzed at various times after onset of the lights and during
the recovery period in the dark. Again, all-trans-retinal was the
only retinoid that accumulated in substantial amounts during
bleaching and recovery. Figure 7 depicts the amount of all-trans-
retinal accumulated during steady state bleaching and during recovery in the dark. The constant light resulted in a
steady state with approximately 35% of the visual pigment
bleached. When the light was turned off, the all-trans-retinal
rapidly decayed to the original dark-adapted value." |
| Comments |
"[Researchers] compared
the rate of decay of all-trans-retinal produced by steady
illumination with that produced by a flash. Approximately the
same amount of visual pigment was bleached in each case. The results, shown in Figure 8, illustrate that the recovery from
steady illumination is approximately 3.5 times more rapid (halflife
[t1/2], 5 minutes with constant illumination. t1/2, 17 minutes
with flash illumination). Similar results were reported in a
study of phosphorylation of rhodopsin.[Oghuro et al., 1995 PMID 7782279]" |
| Entered by |
Uri M |
| ID |
111397 |