| Range |
in the dark ~5% in constant light ~35% %
|
| Organism |
Mouse Mus musculus |
| Reference |
Saari JC. Biochemistry of visual pigment regeneration: the Friedenwald lecture. Invest Ophthalmol Vis Sci. 2000 Feb41(2):337-48. p.340 caption to figure 7PubMed ID10670460
|
| Method |
"Flash illumination of animals and humans has been used
with great success in a number of experimental situations.
However, it could be argued that physiological conditions are
more closely approximated with steady illumination. Thus, [researchers]
thought it important to verify that [their] observation of the
accumulation of all-trans-retinal during recovery from a flash
was not an artifact of the illumination conditions. Dark-adapted
mice were subjected to illumination from two 60-W fluorescent
bulbs (50 foot-candles). Retinoids were extracted and
analyzed at various times after onset of the lights and during
the recovery period in the dark. Again, all-trans-retinal was the
only retinoid that accumulated in substantial amounts during
bleaching and recovery. Figure 7 depicts the amount of all-trans-
retinal accumulated during steady state bleaching and during recovery in the dark. The constant light resulted in a
steady state with approximately 35% of the visual pigment
bleached. When the light was turned off, the all-trans-retinal
rapidly decayed to the original dark-adapted value." |
| Comments |
"FIGURE 7. Recovery from steady state bleaching. The bar at the top of
the figure illustrates the lighting regimen used (filled bar, dark. open
bar, light). In the dark (0 on the abscissa) ~5% of the retinals are in the all-trans configuration. The onset of light increased the amount of
all-trans-retinal to ~40%. When the light was turned off (90 minutes)
all-trans-retinal rapidly decreased to the dark-adapted value." |
| Entered by |
Uri M |
| ID |
111396 |