| Value |
7.5e+5
bcd mRNAs/embryo
Range: ±0.8e+5 bcd mRNAs/embryo
|
| Organism |
Fruit fly Drosophila melanogaster |
| Reference |
Petkova MD, Little SC, Liu F, Gregor T. Maternal origins of developmental reproducibility. Curr Biol. 2014 Jun 2 24(11):1283-8. doi: 10.1016/j.cub.2014.04.028. p.1284 right column bottom paragraph & p.1286 right column 2nd paragraphPubMed ID24856210
|
| Method |
P.1283 right column 3rd paragraph: "To optically identify individual mRNA molecules in whole-mount embryos and to assess embryo-to-embryo reproducibility, [investigators] extended a recently developed mRNA labeling method of fluorescence in situ hybridization (FISH) [refs 18, 20]. [They] labeled bcd mRNAs with synthetic probes and then counted individual molecules and measured their fluorescence intensity by confocal microscopy (Figure 1, Figure S1 available online)." |
| Comments |
p.1284 right column bottom paragraph: "To assign a value for [their] overall estimate, [researchers] averaged the three independent measurements, yielding M(bcd)=(7.5±0.8)×10^5 (SEM). The consistency among the three measures validates the FISH-based counting method in assessing embryo-to embryo bcd mRNA count reproducibility." P.1286 right column 2nd paragraph: "Thus, with M(bcd)=(7.5±0.8)×10^5 mRNA molecules,
[researchers] calculated a translation rate of k≈2 proteins per mRNA per minute, which matches previously reported translation rates during the development of sea urchin embryos [ref 30, Bolouri & Davidson 2003 PMID 12883007]." |
| Entered by |
Uri M |
| ID |
111202 |