Transcription rate in L cells (fibroblast in subcutaneous connective tissue & areolar and adipose tissue)

Range ~2e+8 (prerRNA 39% pre-mRNA (hnRNA) 58% 4–5S RNA 3%) nts/min
Organism Mouse Mus musculus
Reference Jackson DA, Pombo A, Iborra F. The balance sheet for transcription: an analysis of nuclear RNA metabolism in mammalian cells. FASEB J. 2000 Feb14(2):242-54. p.243 right column top paragraphPubMed ID10657981
Primary Source Brandhorst, B. P., and McConkey, E. H. (1974) Stability of nuclear RNA in mammalian cells. J. Mol. Biol. 85, 451–463PubMed ID22003578
Method "Kinetic analyses of the entry of [3H]adenosine into ATP and RNA establish levels of RNA synthesis within intact cells."
Comments "Mouse L cells, which divide every 12–14 h, support a continuous synthetic rate of ~2×10^8 nucleotides/min with 39, 58, and 3% into prerRNA, pre-mRNA (hnRNA), and 4–5S RNA, respectively (primary source)."
Entered by Uri M
ID 111160